ALKALINE PHOSPHATASE ACTIVITY AND pH OPTIMA

Alkaline phosphatase activity and pH optima.

High pH values have been found necessary for optimum activity of alkaline phosphatase in vitro. The pH values in the living cell structures, on the other hand, are near neutrality: cytoplasm 6.9, nucleus 7.6 (l-3). This definite difference between the pH required for optimum activity and the pH of the living cell indicates that alkaline phosphatase is not optimally active in viva. However, the ...

Alkaline inorganic pyrophosphatase activity of mammalian-cell alkaline phosphatase.

Alkaline phosphatase prepared from mammalian cell cultures was found to have alkaline inorganic pyrophosphatase activity. Both of these activities appear to be associated with a single protein, as demonstrated by: (1) concomitant purification of alkaline phosphatase and alkaline inorganic pyrophosphatase; (2) proportional precipitation of alkaline phosphatase and inorganic pyrophosphatase activ...

Skeletal alkaline phosphatase activity in serum.

Alkaline Phosphatase Activity of Leukocytes in Shock

By FRANCESCO VACCARI, BENITO SABOTTO AND ENRICO MANZINI T HE ROLE of the alkaline phosphatases in organic metabolism is riot yet clear. In addition to their role in nucleoprotein metabolism and in the process of ossification, they are also involved in glycogemi synthesis from hmexose diphosphate’ and presumably in gluconeogenesis from proteins.2 Their widespread distribution in the tissues, how...

[Fine structural demonstration of alkaline phosphatase activity].

For the application of Glenner's tetrazolium method to the electron histochemical demonstration of monoamine oxidase some in vitro examination were carried out with special referance to indentification and efflux of formazan and intracellular localization of the enzyme in the locus coeruleus of the rat brain was studied. The results of examinations in vitro are summerized as follows: 1. Formaza...